Physiological function of periplasmic hexose phosphatase in Salmonella typhimurium.
نویسندگان
چکیده
Hydrolysis of sugar phosphates by crude and purified preparations of periplasmic hexose phosphatase from Salmonella typhimurium followed Michaelis-Menten kinetics. The enzyme bound glucose 1-phosphate with high affinity (Km = 10 microM) but bound glucose 6-phosphate with low affinity (Km = 2,000 microM). The order of substrate affinities was glucose 1-phosphate greater than mannose 1-phosphate = galactose 1-phosphate greater than fructose 1-phosphate greater than glucose 6-phosphate. These results and others suggest that the physiological function of the enzyme is the periplasmic hydrolysis of hexose 1-phosphates.
منابع مشابه
Genetic and physiological classification of periplasmic-leaky mutants of Salmonella typhimurium.
Mutants of Salmonella typhimurium that leaked periplasmic proteins were isolated. Four classes of mutants were identified by their increased sensitivity to dyes, detergents, or antibiotics. Conjugation studies indicated that representatives of two classes mapped in the proA-galE region of the Salmonella chromosome and two in the cysI-argE region. According to their bacteriophage sensitivity pat...
متن کاملPeriplasmic nonspecific acid phosphatase II from Salmonella typhimurium LT2. Crystallization, detergent reactivation, and phosphotransferase activity.
The periplasmic nonspecific acid phosphatase II from Salmonella typhimurium was purified to homogeneity from a mutant strain that overproduces the enzyme (Uerkvitz, W., and Beck, C.F. (1981) J. Biol. Chem. 256, 382-389). It was shown that the enzyme transfers phosphate groups from organic phosphoric acid esters (donors) to water as well as to the 2'-, 3'-, or 5'-hydroxyls of nucleosides, nucleo...
متن کاملSynthesis of Escherichia coli uridine diphosphate glucose hydrolase, and demonstration of an inhibitor, in Salmonella typhimurium cytoplasm.
A number of bacterial enzymes, several of which have been shown to be surface-localized, have intracellular protein inhibitors (Swartz et al., 1956; Glaser et al., 1967). The function(s) of these inhibitors has not been identified, though two possibilities have been suggested (Glaser et al., 1967; Hartley, 1974). One way to understand the role of such inhibitors would be to examine a situation ...
متن کاملVirulent Salmonella typhimurium has two periplasmic Cu, Zn-superoxide dismutases.
Periplasmic Cu, Zn-cofactored superoxide dismutase (SodC) protects Gram-negative bacteria from exogenous oxidative damage. The virulent Salmonella typhimurium strain ATCC 14028s has been found to contain two discrete periplasmic Cu, Zn-SOD enzymes that are only 57% identical at the amino acid level. SodCI is carried by a cryptic bacteriophage, and SodCII is closely related to the Cu, Zn-superox...
متن کاملTransport of D-arabinose-5-phosphate and D-sedoheptulose-7-phosphate by the hexose phosphate transport system of Salmonella typhimurium.
d-Arabinose-5-phosphate and d-sedoheptulose-7-phosphate were found to be substrates, although not inducers, of the hexose phosphate transport system of Salmonella typhimurium. Transport of these two sugar phosphates by wild-type strains required preinduction of the hexose phosphate transport system. A mutant of S. typhimurium constitutive for this system also transported d-arabinose-5-phosphate...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 141 3 شماره
صفحات -
تاریخ انتشار 1980